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Rapid test kit one step pcr antigen rapid test kits
Rapid test kit one step pcr antigen rapid test kits

Rapid test kit one step pcr antigen rapid test kits

Principle of Detection】

 1.The complete process of nucleic acid detection: sample collection→nucleic acid extraction→PCR detection.
 2.This product is a multiplex fluorescent probe-based Taqman RT-qPCR assay system. The Taqman fluorescent probe is a specific oligonucleotide based on a reporter-quencher mechanism. For each probe, the 5’-end is labeled with a fluorophore, while the 3’-end was labeled with a quencher. When the probe is intact, the fluorescence emitted by the fluorophore is absorbed by the quencher, and no fluorescent signal is detected. However, during amplification of the template, the probe will be degraded due to the 5'-3’ exonuclease activity of Taq DNA polymerase, and the fluorescent reporter and the quencher are cleaved and separated, then a fluorescent signal can be detected. The generation of each molecular amplicon is accompanied by the generation of a fluorescent signal. Real-time monitoring of the entire PCR process can be assessed by monitoring the accumulation of fluorescent signals.
  • Product Detail

[Product Contents]

Components

Amount(24 tests/kit)

Amount (48 tests/kit)

Ingredient

Detection buffer

240μL

480μL

Buffer、dNTPs

Enzyme Mix

24μL

48μL

RNase Inhibitor,Reverse Transcriptase,Taq DNA polymerase

Primer and Probe Mix

48μL

96μL

Primers, Probes

Positive Control

500μL

500μL

RNA pseudovirus containing target gene

Negative Control

500μL

500μL

ddH2O

[Adventage]

    • Internal control: Human􀁔-Globin gene as the internal control is included into the reagent to  verify the validity of the experiment.
      * High sensitivity: The lowest detection limit is 250 copies/ml
      * High specificity: Primers and probes are designed for spec巾c fragments of two gene regions, which confirm each other to make the results more accurate. No cross-reactivity with other pathogens with the same site of infection or s吓ilar infection characteristics
      * Strong stab山ty: CV of each channel is all <3%
      * Multiple Real-time RT-PCR detection: Each channel does not interfere with each other, and the amplification curve is S-shaped.
    • * Simple operation: one-step method to complete RT-PCR, The whole procedure can be detected within 80min.
      * Fast speed: The PCR amplification time is less than 80 minutes.

[Note]

  1. Do not mix the components from different batches for detection.
  2. Additional Materials Required: Nucleic acid extraction reagents.The instrument used for extraction kit should be selected according to the instruction

  3. Nucleic acid extraction must be performed simultaneously with the Positive control (2019-nCoV-pseudoviruse) and Negative Control (ddH2O) formonitoring the entire procedure to reduce false negative or false positive rates.

About Us

FAQ

1.Are you factory/manufacturer or trade company?

Yes,We are the direct factory manufacturer who owns productsion lines and workers.and everything is flexible and you don't worry about changing extra money by the middle man or trader.

2.Can you make our design ?

Yes,Your own designs/sketches/pictures are welcoming.

3.Samples available?

Yes,Samples day 3-7days ,more daysif make complicate design .

4.Shipment?

Samples use fast express(UPS,DHL,FEDEX,etc),bulk delivery by air or ship.

5.Payment Terms?

Western Union and T/T,Alipay ,For your convenience.

 

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